Overview

  • Product nameAnti-FUT3 antibody
    See all FUT3 primary antibodies
  • Description
    Rabbit polyclonal to FUT3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal sequence of FUT3.

  • Positive control
    • Human colon carcinoma tissue; extracts from K562 cells and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
  • Tissue specificityHighly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 10 family.
  • Cellular localizationGolgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-(1,3/1,4)-fucosyltransferase antibody
    • Blood group Lewis alpha-4-fucosyltransferase antibody
    • CD174 antibody
    • FT3B antibody
    • Fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) antibody
    • Fucosyltransferase 3 antibody
    • Fucosyltransferase III antibody
    • FucT III antibody
    • FucT-III antibody
    • FUT3 antibody
    • FUT3_HUMAN antibody
    • Galactoside 3(4)-L-fucosyltransferase antibody
    • LE antibody
    • Les antibody
    • Lewis FT antibody
    see all

Anti-FUT3 antibody images

  • All lanes : Anti-FUT3 antibody (ab110082) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
  • Immunohistochemical analysis of Blood Group Lewis b in paraffin embedded Human colon carcinoma tissue, using a 1/50 dilution of ab110082 with (right image) or without (left image) immunizig peptide.

References for Anti-FUT3 antibody (ab110082)

ab110082 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (breast cancer MDA-MB-231)
Specification breast cancer MDA-MB-231
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"