Overview

  • Product name
  • Description
    Rabbit polyclonal to FUT3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal sequence of FUT3.

  • Positive control
    • Human colon carcinoma tissue; extracts from K562 cells and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
  • Tissue specificity
    Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 10 family.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-(1,3/1,4)-fucosyltransferase antibody
    • Blood group Lewis alpha-4-fucosyltransferase antibody
    • CD174 antibody
    • FT3B antibody
    • Fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) antibody
    • Fucosyltransferase 3 antibody
    • Fucosyltransferase III antibody
    • FucT III antibody
    • FucT-III antibody
    • FUT3 antibody
    • FUT3_HUMAN antibody
    • Galactoside 3(4)-L-fucosyltransferase antibody
    • LE antibody
    • Les antibody
    • Lewis FT antibody
    see all

Images

  • All lanes : Anti-FUT3 antibody (ab110082) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
  • Immunohistochemical analysis of Blood Group Lewis b in paraffin embedded Human colon carcinoma tissue, using a 1/50 dilution of ab110082 with (right image) or without (left image) immunizig peptide.

References

ab110082 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (breast cancer MDA-MB-231)
Specification
breast cancer MDA-MB-231
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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