Overview

  • Product name
  • Description
    Rabbit polyclonal to FUT6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 110-140 of Human FUT6

  • Positive control
    • HL-60 cell line lysates, bladder carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab104270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Enzyme involved in the biosynthesis of the E-Selectin ligand, sialyl-Lewis X. Catalyzes the transfer of fucose from GDP-beta-fucose to alpha-2,3 sialylated substrates.
  • Cellular localization
    Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
  • Database links
  • Alternative names
    • Alpha (1,3) fucosyltransferase antibody
    • EC=2.4.1.65 antibody
    • FCT3A antibody
    • FT1A antibody
    • Fuc TVI antibody
    • fucosyltransferase 6 (alpha (1,3) fucosyltransferase) antibody
    • Fucosyltransferase 6 antibody
    • Fucosyltransferase VI antibody
    • FucT VI antibody
    • Galactoside 3 L fucosyltransferase antibody
    see all

Images

  • Anti-FUT6 antibody (ab104270) at 1/100 dilution + HL-60 cell line lysates at 35 µg

    Predicted band size : 42 kDa
  • IHC analysis of FUT6 expression in formalin fixed and paraffin embedded bladder carcinoma using 1/50 ab104270 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab104270 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100000 cells
Specification
breast cancer
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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