Overview

  • Product nameAnti-FUZ antibody
    See all FUZ primary antibodies
  • Description
    Rabbit polyclonal to FUZ
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LLRNFYTLVT STHFPPEPGP PEKTEDEVYQ AQLPRACYLV LGTEEPGTGV RLVALQLGLR RLLLLLSPQS PTHGLRSLAT HTLHALTP, corresponding to amino acids 329-416 of Human FUZ.

  • Positive control
    • Human smooth muscle tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • FunctionProbable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. May regulate the morphogenesis of hair follicles which depends on functional primary cilia.
  • Sequence similaritiesBelongs to the fuzzy family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22688 antibody
    • fuz antibody
    • FUZZY_HUMAN antibody
    • Fy antibody
    • Protein fuzzy homolog antibody
    see all

Anti-FUZ antibody images

  • Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.
  • ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References for Anti-FUZ antibody (ab122742)

ab122742 has not yet been referenced specifically in any publications.

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Under our Abpromise we guarantee both ab122778 and ab122742 to work in IF on human samples. If you were to purchase either of them and they were not to perform as stated, then we would be happy to either replac...

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