Overview

  • Product name
  • Description
    Rabbit polyclonal to FXYD1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human FXYD1.

    (Peptide available as ab88161.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Brain Membrane; Human Heart; Human Skeletal Muscle

Properties

Applications

Our Abpromise guarantee covers the use of ab76597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.
  • Cellular localization
    Membrane; single-pass type I membrane protein
  • Database links
  • Alternative names
    • FXYD domain containing ion transport regulator 1 antibody
    • Phospholemman antibody
    • PLM antibody

Anti-FXYD1 antibody images

  • All lanes : Anti-FXYD1 antibody (ab76597) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa
    Observed band size : 10 kDa


    Exposure time : 5 minutes
  • ab76597 at a 1/100 dilution, staining mouse FXYD1 in heart and muscle tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

References for Anti-FXYD1 antibody (ab76597)

This product has been referenced in:
  • Wanichawan P  et al. Development of a high-affinity peptide that prevents phospholemman (PLM) inhibition of the sodium/calcium exchanger 1 (NCX1). Biochem J 473:2413-23 (2016). WB ; Rat . Read more (PubMed: 27247424) »
  • Howie J  et al. Substrate recognition by the cell surface palmitoyl transferase DHHC5. Proc Natl Acad Sci U S A 111:17534-9 (2014). Read more (PubMed: 25422474) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Human Cardiomyocytes)
Loading amount
20 µg
Specification
Human Cardiomyocytes
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

Submitted Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse cardiomyocyte)
Loading amount
20 µg
Specification
Mouse cardiomyocyte
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

Submitted Apr 27 2010

Application
Western blot
Sample
Rat Tissue lysate - whole (Rat cardiomyocyte)
Loading amount
20 µg
Specification
Rat cardiomyocyte
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

Submitted Apr 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart, muscle)
Specification
heart, muscle
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, Intestine)
Loading amount
20 µg
Specification
Brain, Intestine
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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