The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
Use at an assay dependent concentration.
PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.
FXYD domain containing ion transport regulator 1 antibody
Western blot - Anti-FXYD1 antibody (ab76597)
All lanes : Anti-FXYD1 antibody (ab76597) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456) Lane 3 : Human heart tissue lysate - total protein (ab29431) Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa Observed band size: 10 kDa
Wanichawan P et al. Development of a high-affinity peptide that prevents phospholemman (PLM) inhibition of the sodium/calcium exchanger 1 (NCX1). Biochem J473:2413-23 (2016).
Read more (PubMed: 27247424) »
Hafver TL et al. Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman. J Biol Chem291:4561-79 (2016).
Read more (PubMed: 26668322) »