Recombinant Anti-FXYD1/PLM antibody [EPR16082] (ab200204)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16082] to FXYD1/PLM
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FXYD1/PLM antibody [EPR16082]
See all FXYD1/PLM primary antibodies -
Description
Rabbit monoclonal [EPR16082] to FXYD1/PLM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle, fetal heart and fetal liver lysates; Mouse and rat heart lysates. IP: Human fetal heart whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16082 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
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IP |
1/120.
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Notes |
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WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). |
IP
1/120. |
Target
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Function
May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. -
Tissue specificity
Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis. -
Sequence similarities
Belongs to the FXYD family. -
Post-translational
modificationsMajor plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5348 Human
- Entrez Gene: 56188 Mouse
- Entrez Gene: 58971 Rat
- Omim: 602359 Human
- SwissProt: O00168 Human
- SwissProt: Q9Z239 Mouse
- SwissProt: O08589 Rat
- Unigene: 442498 Human
see all -
Alternative names
- FXYD domain containing ion transport regulator 1 antibody
- FXYD domain-containing ion transport regulator 1 antibody
- FXYD1 antibody
see all
Images
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Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/10000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal heart whole cell lysate 10ug (Input).
Lane 2: ab200204 IP in Human fetal heart whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab200204 has been referenced in 1 publication.
- Eleftheriadou O et al. Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy. Basic Res Cardiol 112:37 (2017). Rat . PubMed: 28526910