Anti-FYB antibody [EPR2547Y] (ab76006)

Overview

  • Product nameAnti-FYB antibody [EPR2547Y]
    See all FYB primary antibodies
  • Description
    Rabbit monoclonal [EPR2547Y] to FYB
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide designed in the C-terminus of Human FYB

  • Positive control
    • TF-1 lysate. Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notesICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/500. Predicted molecular weight: 85 kDa.

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.
    • Tissue specificityExpressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
    • Sequence similaritiesContains 1 SH3 domain.
    • Post-translational
      modifications
      T-cell receptor ligation leads to increased tyrosine phosphorylation.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADAP antibody
      • Adhesion and degranulation promoting adaptor protein antibody
      • FYB 120/130 antibody
      • Fyb antibody
      • FYB-120/130 antibody
      • FYB_HUMAN antibody
      • FYN binding protein antibody
      • FYN T binding protein antibody
      • FYN-binding protein antibody
      • FYN-T-binding protein antibody
      • p120/p130 antibody
      • PRO0823 antibody
      • SLAP 130 antibody
      • SLAP-130 antibody
      • SLAP130 antibody
      • SLP 76 associated phosphoprotein antibody
      • SLP-76-associated phosphoprotein antibody
      • SLP76 associated phosphoprotein antibody
      see all

    Anti-FYB antibody [EPR2547Y] images

    • Anti-FYB antibody [EPR2547Y] (ab76006) at 1/500 dilution + TF-1 lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 85 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • ab76006, at 1/100 dilution, staining FYB in human tonsil by immunohistochemistry using paraffin-embedded tissue.

    References for Anti-FYB antibody [EPR2547Y] (ab76006)

    ab76006 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (CHO cell transfected)
    Loading amount 20 µg
    Specification CHO cell transfected
    Gel Running Conditions Reduced Denaturing (7.5%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"