Overview

  • Product name
  • Description
    Goat polyclonal to Fyn
  • Specificity
    This antibody is expected to recognise all three reported isoforms (as represented by NP_002028; NP_694592; NP_694593).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Fyn aa 3-15 (N terminal).
    Sequence:

    VQCKDKEATKLTE


    (Peptide available as ab22784)

  • Positive control
    • Jurkat, A431 and NIH3T3 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 64 kDa).Can be blocked with Human Fyn peptide (ab22784).

Target

  • Function
    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • Tissue specificity
    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt
  • Database links
  • Form
    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

Images

  • All lanes : Anti-Fyn antibody (ab802) at 0.3 µg/ml

    Lane 1 : A431 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 64 kDa

References

ab802 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely offer free samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information a...

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This antibody has not been tested for suitability in IHC on formalin fixed paraffin embedded sections, therefore we can't guarantee results.

Although suitable for western blotting, this antibody has not been tested on rabbit samples, so we can't guarantee results.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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