Overview

  • Product name
    Anti-Fyn antibody [FYN-01]
    See all Fyn primary antibodies
  • Description
    Mouse monoclonal [FYN-01] to Fyn
  • Specificity
    The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.
  • Tested applications
    Suitable for: IP, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.

Properties

Applications

Our Abpromise guarantee covers the use of ab1881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa.

Target

  • Function
    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • Tissue specificity
    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt
  • Database links
  • Form
    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

Images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa


    Exposure time : 10 seconds
  • Immunocytochemistry/ Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 µL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue). 
  • Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Suo M  et al. Role of Fyn-mediated NMDA receptor function in prediabetic neuropathy in mice. J Neurophysiol 116:448-55 (2016). Read more (PubMed: 27146985) »
  • Higa-Nakamine S  et al. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem 290:25974-85 (2015). Read more (PubMed: 26338704) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry.
The image on the datasheet of ab1881 shows a WB with the positive control ab84696.
ab84696 is a Active human Fyn full length protein that has a tag. The tag has a size of ca. 26kDa.
Therefore the band at ca 85...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and r...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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