Fyn overexpression 293T lysate (whole cell) (ab94223)

Overview

  • Product nameFyn overexpression 293T lysate (whole cell)
  • Description
    Fyn overexpression lysate
  • General notesab94223 is a 293T cell transfected lysate in which Human Fyn has been transiently over-expressed using a pCMV-Fyn plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. Tissue specificity: Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Fyn overexpression 293T lysate (whole cell) images

  • ab94223 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Fyn antibody (ab76934) at 1/500 dilution

    Lane 1 : Fyn overexpression 293T lysate (whole cell) (ab94223)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Fyn overexpression 293T lysate (whole cell) (ab94223)

ab94223 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"