Overview

  • Product name
  • Description
    Rabbit polyclonal to FYTTD1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 34-74 of Human FYTTD1.

  • Positive control
    • A375 cell lysate; Mouse liver tissue lysate; Human cervix carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab103722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for mRNA export from the nucleus to the cytoplasm. Acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. Associates with spliced and unspliced mRNAs simultaneously with THOC4.
  • Sequence similarities
    Belongs to the UIF family.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761B1514 antibody
    • Forty two three domain containing 1 antibody
    • Forty two three domain containing protein 1 antibody
    • Forty-two-three domain-containing protein 1 antibody
    • FYTTD 1 antibody
    • fyttd1 antibody
    • OTTHUMP00000208028 antibody
    • OTTHUMP00000208031 antibody
    • OTTHUMP00000208032 antibody
    • Protein 40 2 3 antibody
    • Protein 40-2-3 antibody
    • UAP56 interacting factor antibody
    • UAP56-interacting factor antibody
    • UIF antibody
    • UIF_HUMAN antibody
    see all

Images

  • Anti-FYTTD1 antibody (ab103722) at 1/100 dilution + A375 cell lysate at 35 µg

    Predicted band size : 36 kDa
  • Anti-FYTTD1 antibody (ab103722) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 36 kDa
  • ab103722, at 1/50 dilution, staining FYTTD1 in formalin-fixed, paraffin-embedded Human cervix carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References

ab103722 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
20000 cells
Specification
293T cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293T cells)
Specification
293T cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% triton x100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Nov 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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