Overview

  • Product nameAnti-FZD3 antibody
    See all FZD3 primary antibodies
  • Description
    Rabbit polyclonal to FZD3
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human FZD3.

  • Positive control
    • HUVEC cell extracts. A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 76 kDa.
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificityWidely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationMembrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled 3 antibody
    • Frizzled 3 seven transmembrane spanning receptor antibody
    • Frizzled family receptor 3 antibody
    • Frizzled homolog 3 (Drosophila) antibody
    • Frizzled homolog 3 antibody
    • Frizzled-3 antibody
    • Frizzled3 antibody
    • Fz 3 antibody
    • Fz-3 antibody
    • Fz3 antibody
    • FZD 3 antibody
    • Fzd3 antibody
    • FZD3_HUMAN antibody
    • hFz 3 antibody
    • hFz3 antibody
    see all

Anti-FZD3 antibody images

  • ab75233 at 1/500 dilution staining FZD3 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-FZD3 antibody (ab75233) at 1/500 dilution

    Lane 1 : HUVEC cell extracts
    Lane 2 : HUVEC cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 76 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 117 kDa,34 kDa. We are unsure as to the identity of these extra bands.

References for Anti-FZD3 antibody (ab75233)

ab75233 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Specification E9.5 mouse embryo section
Permeabilization Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative Paraformaldehyde
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Submitted Jan 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"