Overview

  • Product name
  • Description
    Rabbit polyclonal to FZR1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 (SSPVSSPSKH GDRFIPSRAG ANWSVNFHRI NENEKSPSQN RKAKDATSDN) of Human FZR1 (NP_057347; UniProt Q9UM11 isoform 2).

  • Positive control
    • 721_B cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
  • Tissue specificity
    Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC20 like 1b antibody
    • CDC20 like protein 1 antibody
    • CDC20-like protein 1 antibody
    • CDC20C antibody
    • CDH1 antibody
    • Cdh1/Hct1 homolog antibody
    • Fizzy related protein 1 antibody
    • Fizzy related protein antibody
    • Fizzy related protein homolog antibody
    • Fizzy-related protein homolog antibody
    • Fizzy/cell division cycle 20 related 1 antibody
    • FYR antibody
    • FZR 1 antibody
    • FZR 2 antibody
    • Fzr antibody
    • FZR_HUMAN antibody
    • Fzr1 antibody
    • Fzr1 protein antibody
    • FZR2 antibody
    • HCDH 1 antibody
    • HCDH antibody
    • hCDH1 antibody
    • KIAA1242 antibody
    see all

Images

  • Anti-FZR1 antibody (ab118939) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 55 kDa

References

ab118939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab118939.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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