Overview

  • Product name
    Anti-FZR1 antibody [AR38.2]
    See all FZR1 primary antibodies
  • Description
    Mouse monoclonal [AR38.2] to FZR1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    AR38.2
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
  • Tissue specificity
    Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC20 like 1b antibody
    • CDC20 like protein 1 antibody
    • CDC20-like protein 1 antibody
    • CDC20C antibody
    • CDH1 antibody
    • Cdh1/Hct1 homolog antibody
    • Fizzy related protein 1 antibody
    • Fizzy related protein antibody
    • Fizzy related protein homolog antibody
    • Fizzy-related protein homolog antibody
    • Fizzy/cell division cycle 20 related 1 antibody
    • FYR antibody
    • FZR 1 antibody
    • FZR 2 antibody
    • Fzr antibody
    • FZR_HUMAN antibody
    • Fzr1 antibody
    • Fzr1 protein antibody
    • FZR2 antibody
    • HCDH 1 antibody
    • HCDH antibody
    • hCDH1 antibody
    • KIAA1242 antibody
    see all

Images

  • All lanes : Anti-FZR1 antibody [AR38.2] (ab77885) at 2.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Murine myeloid cells


    Predicted band size : 55 kDa

References

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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