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Anti-FZR1 antibody [DH01 (DCS-266)] (ab3242)

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Overview

Product name

Anti-FZR1 antibody [DH01 (DCS-266)]
See all FZR1 products (5) ...

Description

Mouse monoclonal [DH01 (DCS-266)] to FZR1

Tested applications

IP, WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant full length protein (Human).

Positive control

Jurkat or LS174T cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS with 0.2% BSA, 0.09% sodium azide, pH7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

DH01 (DCS-266)

Isotype

IgG1

Light chain type

kappa

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C

  • Flow Cytometry-FZR1 antibody [DH01 (DCS-266)](ab3242)Flow Cytometry-FZR1 antibody [DH01 (DCS-266)](ab3242) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Tissue specificity

Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats.

Post-translational
modifications

Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.

Cellular localization

Cytoplasm and Nucleus.

Target information above from: UniProt accessionQ9UM11 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CDC20 like 1b antibody
  • CDC20 like protein 1 antibody
  • CDC20-like protein 1 antibody
  • CDC20C antibody
  • Cdh 1 antibody
  • Cdh1/Hct1 homolog antibody
  • Fizzy related protein 1 antibody
  • Fizzy related protein 1 antibody
  • Fizzy related protein antibody
  • Fizzy related protein homolog antibody
  • Fizzy-related protein homolog antibody
  • Fizzy/cell division cycle 20 related 1 antibody
  • FYR antibody
  • FZR 1 antibody
  • FZR 2 antibody
  • FZR antibody
  • FZR_HUMAN antibody
  • FZR1 antibody
  • Fzr1 protein antibody
  • FZR2 antibody
  • HCDH 1 antibody
  • HCDH antibody
  • HCDH1 antibody
  • KIAA1242 antibody
see all

Database links

see all

Anti-FZR1 antibody [DH01 (DCS-266)] images:

  Flow Cytometry-FZR1 antibody [DH01 (DCS-266)](ab3242)

Flow Cytometry-FZR1 antibody [DH01 (DCS-266)](ab3242)

Overlay histogram showing HeLA cells stained with ab3242 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3242, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-FZR1 antibody [DH01 (DCS-266)] (ab3242)

This product has been referenced in:

  • Chen M  et al. The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress. PLoS One 7:e35520 (2012). WB; Human.Read more (PubMed: 22539978) »
  • Holt JE  et al. The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes. Development 138:905-13 (2011).Read more (PubMed: 21270054) »

See all 11 publications for this product

Publishing research using ab3242? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"