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Anti-GAB1 antibody (ab47513)

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Overview

Product name

Anti-GAB1 antibody
See all GAB1 products (10) ...

Description

Rabbit polyclonal to GAB1

Specificity

Detects endogenous levels of total GAB1 protein.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide: VEYLD, corresponding to amino acids 625-629 of Human GAB1 - around the phosphorylation site of Tyrosine 627.

VEYLD

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human umbilical vein endothelial cells.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Affinity purified from rabbit antiserum by affinity chromatpgraphy using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases

  • Western blot - GAB1 antibody (ab47513)Western blot - GAB1 antibody (ab47513) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GAB1 antibody(ab47513)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GAB1 antibody(ab47513) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 118 kDa (predicted molecular weight: 77 kDa).

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Function

Probably involved in EGF and insulin receptor signaling.

Sequence similarities

Belongs to the GAB family.
Contains 1 PH domain.

Post-translational
modifications

Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.

Target information above from: UniProt accessionQ13480 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Gab 1 antibody
  • Gab1 antibody
  • GAB1_HUMAN antibody
  • GRB 2 associated binder 1 antibody
  • GRB 2 associated binding protein 1 antibody
  • GRB2 associated binding protein 1 isoform a antibody
  • GRB2 associated binding protein 1 isoform b antibody
  • GRB2-associated binder 1 antibody
  • GRB2-associated-binding protein 1 antibody
  • Growth factor receptor bound protein 2-associated protein 1 antibody
see all

Database links

Anti-GAB1 antibody images:

  Western blot - GAB1 antibody (ab47513)

Western blot - GAB1 antibody (ab47513)

All lanes : Anti-GAB1 antibody (ab47513)

Lane 1 : HUVEC cell extract treated with EGF (200ng/ml, 10 min)
Lane 2 : HUVEC cell extract treated with EGF (200ng/ml, 10 min) with peptide

Lysates/proteins at 30 µg per lane.


Predicted band size : 77 kDa
Observed band size : 118 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GAB1 antibody(ab47513)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GAB1 antibody(ab47513)

Ab47513 staining human normal tonsil. Staining is localised to the cytoplasm and cell membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-GAB1 antibody (ab47513)

ab47513 has not yet been referenced specifically in any publications.

Publishing research using ab47513? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"