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Western blot - GAB1 antibody (ab47513)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GAB1 antibody(ab47513)
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Product Name
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GAB1 antibody
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See all GAB1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GAB1
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Immunogen
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Synthetic peptide: VEYLD, corresponding to amino acids 625-629 of Human GAB1 - around the phosphorylation site of Tyrosine 627.
BLAST 'VEYLD' with  or 
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Detects endogenous levels of total GAB1 protein.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 77 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human umbilical vein endothelial cells.
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Cellular localization
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Cytoplasmic (probable).
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Research areas
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Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Affinity purified from rabbit antiserum by affinity chromatpgraphy using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GAB1 antibody images, references, products related to ab47513 and other tools.
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GAB1 antibody images:
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 Western blot - GAB1 antibody (ab47513)
All lanes : GAB1 antibody (ab47513)
Lane 1 : HUVEC cell extract treated with EGF (200ng/ml, 10 min)
Lane 2 : HUVEC cell extract treated with EGF (200ng/ml, 10 min) with peptide
Lysates/proteins at 30 µg per lane.
Predicted band size : 77 kDa
Observed band size : 118 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GAB1 antibody(ab47513)
Ab47513 staining human normal tonsil. Staining is localised to the cytoplasm and cell membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to GAB1
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GAB1 antibody - more information
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Customer reviews (feedback) regarding GAB1 antibody |
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