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Human recombinant granulocyte-colony stimulating factor.
The neutralising bioassay was performed on myeloid leukemia G-NFS-60 cell line. G-CSF stimulates the proliferation of these cells in vitro. Briefly, human G-CSF was pre-incubated with monoclonal antibody 5D7 for 2 h at RT and then added to the cell suspension. Cells were cultured for 72 h. Cell proliferation was measured by MTT method. Polyclonal antibody to G-CSF and an irrelevant monoclonal antibody of the same isotype were used as controls.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR241046-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab9818 in the following tested applications.
|WB||1/1000 - 1/5000.|
|Sandwich ELISA||Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to G-CSF (ab9691). For sandwich ELISA, use this antibody as Capture at 1µg/ml>ab9691 as Detection.|
|Indirect ELISA||1/2000 - 1/20000.|
ab9818 has not yet been referenced specifically in any publications.