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Human recombinant granulocyte-colony stimulating factor.
The neutralising bioassay was performed on myeloid leukemia G-NFS-60 cell line. G-CSF stimulates the proliferation of these cells in vitro. Briefly, human G-CSF was pre-incubated with monoclonal antibody 5D7 for 2 h at RT and then added to the cell suspension. Cells were cultured for 72 h. Cell proliferation was measured by MTT method. Polyclonal antibody to G-CSF and an irrelevant monoclonal antibody of the same isotype were used as controls.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR241046-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab9818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|Sandwich ELISA||Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to G-CSF (ab9691). For sandwich ELISA, use this antibody as Capture at 1µg/ml>ab9691 as Detection.|
|Indirect ELISA||1/2000 - 1/20000.|
ab9818 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"