Anti-G-CSF antibody [67604.111] (ab49960)


  • Product nameAnti-G-CSF antibody [67604.111]
    See all G-CSF primary antibodies
  • Description
    Rat monoclonal [67604.111] to G-CSF
  • Specificityab49960 neutralizes the biological activity of recombinant mouse G-CSF.
  • Tested applicationsSuitable for: IHC-P, WB, Neutralising, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Purified recombinant mouse G-CSF, expressed in E. coli.

  • Positive control
    • Mouse G-CSF
  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method



Our Abpromise guarantee covers the use of ab49960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
  • Application notesI-ELISA: Use at a concentration of 0.5 - 1 µg/ml.
    Neut: Use at a concentration of 10 - 30 mg/ml will neutralize 50% of the bioactivity due to 0.125 ng/ml recombinant mouse G-CSF using NSF-60 cells.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
    • Sequence similaritiesBelongs to the IL-6 superfamily.
    • Post-translational
      O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C17orf33 antibody
      • Colony stimulating factor 3 (granulocyte) antibody
      • Colony stimulating factor 3 antibody
      • CSF 3 antibody
      • CSF beta antibody
      • CSF3 antibody
      • CSF3_HUMAN antibody
      • CSF3OS antibody
      • Csfg antibody
      • Filgrastim antibody
      • G-CSF antibody
      • GCSA antibody
      • GCSF antibody
      • Granulocyte colony stimulating factor antibody
      • Granulocyte colony-stimulating factor antibody
      • Lenograstim antibody
      • Macrophage granulocyte inducer 2 antibody
      • MGC45931 antibody
      • MGI 2 antibody
      • Pluripoietin antibody
      see all

    Anti-G-CSF antibody [67604.111] images

    • ab49960 staining G-CSF in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 7.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris-base buffer. Samples were incubated with primary antibody (1/90 in PBST) for 3 hours at 25°C. A HRP-conjugated Goat anti-rat IgG polyclonal (1/90) was used as the secondary antibody.

      See Abreview

    References for Anti-G-CSF antibody [67604.111] (ab49960)

    ab49960 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Bone marrow-derived macrophage)
    Permeabilization Yes - Intracellular Staining Permeabilization Wash Buffer (biolegend), 0.5% Triton-X
    Specification Bone marrow-derived macrophage
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Fixation Buffer (biolegend)

    Abcam user community

    Verified customer

    Submitted Feb 23 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-base Buffer
    Sample Mouse Tissue sections (Skin)
    Specification Skin
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 7.5% · Temperature: 25°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 01 2013

    Thank you for your patience in awaiting our reply. I have checked all of our monoclonal G-CSF antibodies and none of them have been epitope mapped, so we do not know where they bind. The polyclonal antibodies, of course, will bind many places. Please l...

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