Anti-G-CSF antibody (ab231277)
Key features and details
- Rabbit polyclonal to G-CSF
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-G-CSF antibody
See all G-CSF primary antibodies -
Description
Rabbit polyclonal to G-CSF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Pig G-CSF aa 1 to the C-terminus. (Expressed in E.coli).
Database link: O02837 -
Positive control
- WB: Human leukocyte cell lysate; Mouse lymphocyte cell lysate: Mouse thymus tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Followed by Protein A chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 22 kDa.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 22 kDa. |
Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1440 Human
- Entrez Gene: 12985 Mouse
- Omim: 138970 Human
- SwissProt: P09919 Human
- SwissProt: P09920 Mouse
- SwissProt: O02837 Pig
- Unigene: 2233 Human
- Unigene: 1238 Mouse
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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All lanes : Anti-G-CSF antibody (ab231277) at 2 µg/ml
Lane 1 : Mouse lymphocyte cells
Lane 2 : Human leukocyte cells
Lane 3 : Mouse thymus tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab. at 1/2000 dilution
Predicted band size: 22 kDa -
Anti-G-CSF antibody (ab231277) at 2 µg/ml + Recombinant pig G-SCF protein.
Predicted band size: 22 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231277 has not yet been referenced specifically in any publications.