Anti-G-CSF antibody (ab9691)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hG-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hG-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab9818 as Capture.
IHC-P Use a concentration of 0.25 µg/ml.
Flow Cyt 1/300.

Target

  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C17orf33 antibody
    • Colony stimulating factor 3 (granulocyte) antibody
    • CSF 3 antibody
    • CSF beta antibody
    • CSF3 antibody
    • CSF3_HUMAN antibody
    • CSF3OS antibody
    • Csfg antibody
    • Filgrastim antibody
    • G-CSF antibody
    • GCSA antibody
    • GCSF antibody
    • Granulocyte colony stimulating factor antibody
    • Granulocyte colony-stimulating factor antibody
    • Lenograstim antibody
    • Macrophage granulocyte inducer 2 antibody
    • MGC45931 antibody
    • MGI 2 antibody
    • Pluripoietin antibody
    see all

Anti-G-CSF antibody images

  • Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml
  • ab9691 staining G-CSF in human angiosarcoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • developed using the ECL technique

    Performed under reducing conditions.
  • ab9691 staining G-CSF in Human HL-1 cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/300) for 1 hour at 20°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Gating Strategy: Isotype population (shown in white).

    See Abreview

References for Anti-G-CSF antibody (ab9691)

ab9691 has not yet been referenced specifically in any publications.

Product Wall

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.



There is no real expiry date on all our antibodies. Usually the life is 10 years if stored in -20. Minimal freeze thaw cycles definitely extend the life of the antibody.

Thank you for contacting us. We do not currently have that particular lot in stock but we do have lots of antibody produced from the same bleed and purification. The only difference would be that the antibody was aliquotted by us at a different time. T...

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Thank you for your call today and for your question.

We don't assign specific expiration dates for our products (our guarantee is for 6 months after purchase), but we expect an antibody stored at -20C to be viable for up to 5 years.

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Thank you for contacting. Please find attached the Certificate of Analysis for ab9691. If there is anything else I can help you with, please let me know.

Thank you for your enquiry. Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abprom...

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There are various experiments that can demonstrate that an antibody binds specifically to the protein it was generated against, for instance western blotting or ELISA of a complex mixture of proteins that contains the protein of interest, but these ass...

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Thank you for contacting us. If you are seeing an effect with the antibodies compared to absence of antibody, then another control to compare is an isotype control. For ab9691, this would be rabbit IgG with no known specificity, for instance ab...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (E.coli)
Loading amount 3 µg
Specification E.coli
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Chul sOO Cheong

Verified customer

Submitted Feb 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"