Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hG-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hG-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab9818 as Capture.
IHC-P Use a concentration of 0.25 µg/ml.
Flow Cyt 1/300. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C17orf33 antibody
    • Colony stimulating factor 3 (granulocyte) antibody
    • Colony stimulating factor 3 antibody
    • CSF 3 antibody
    • CSF beta antibody
    • CSF3 antibody
    • CSF3_HUMAN antibody
    • CSF3OS antibody
    • Csfg antibody
    • Filgrastim antibody
    • G-CSF antibody
    • GCSA antibody
    • GCSF antibody
    • Granulocyte colony stimulating factor antibody
    • Granulocyte colony-stimulating factor antibody
    • Lenograstim antibody
    • Macrophage granulocyte inducer 2 antibody
    • MGC45931 antibody
    • MGI 2 antibody
    • Pluripoietin antibody
    see all

Images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
  • ab9691 staining G-CSF in human angiosarcoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
  • Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml

  • Developed using the ECL technique

    Performed under reducing conditions.

References

This product has been referenced in:
  • Makino T  et al. Diffuse fluorodeoxyglucose-positron uptake in the bone marrow of a patient with granulocyte colony-stimulating factor-producing pleomorphic carcinoma of the lung: A case report. Mol Clin Oncol 7:103-106 (2017). Read more (PubMed: 28685085) »
  • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). IHC-P ; Human . Read more (PubMed: 27141367) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
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Submitted May 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

Submitted May 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Formalin
Username

Abcam user community

Verified customer

Submitted May 19 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
Username

Abcam user community

Verified customer

Submitted May 19 2017

Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Cell lysate - whole cell (PC-12 cells)
Specification
PC-12 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Jun 17 2013

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.



There is no real expiry date on all our antibodies. Usually the life is 10 years if stored in -20. Minimal freeze thaw cycles definitely extend the life of the antibody.

Thank you for contacting us. We do not currently have that particular lot in stock but we do have lots of antibody produced from the same bleed and purification. The only difference would be that the antibody was aliquotted by us at a different time. T...

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Thank you for your call today and for your question.

We don't assign specific expiration dates for our products (our guarantee is for 6 months after purchase), but we expect an antibody stored at -20C to be viable for up to 5 years.

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Thank you for contacting. Please find attached the Certificate of Analysis for ab9691. If there is anything else I can help you with, please let me know.

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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