Human G-CSF ELISA Kit (ab100524)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.69 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human G-CSF ELISA Kit
See all G-CSF kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
0.69 pg/ml - 500 pg/ml -
Recovery
> 75 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.56 68% - 90% Serum 83.45 72% - 94% Plasma 74.76 67% - 87% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 260X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human G-CSF 2 vials Human G-CSF Microplate (12 x 8 wells) 1 unit Recombinant Human G-CSF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions. -
Alternative names
- C17orf33
- Colony stimulating factor 3 (granulocyte)
- CSF 3
see all -
Database links
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
Images
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Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).
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Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).
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Representative standard curve using ab100524
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G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).
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Representative standard curve using ab100524
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100524 has been referenced in 2 publications.
- Andar A et al. Wood Microfluidics. Anal Chem 91:11004-11012 (2019). PubMed: 31361950
- Zhang W et al. Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle. Bosn J Basic Med Sci N/A:N/A (2019). PubMed: 31215856