Anti-G protein alpha 16 antibody (ab154157)

Overview

  • Product name
    Anti-G protein alpha 16 antibody
    See all G protein alpha 16 primary antibodies
  • Description
    Rabbit polyclonal to G protein alpha 16
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 112-374 of Human G protein alpha 16 (UniProt ID: P30679).

  • Positive control
    • THP1 whole cell lysate; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab154157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
  • Tissue specificity
    Specifically expressed in hematopoietic cells.
  • Sequence similarities
    Belongs to the G-alpha family. G(q) subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • G alpha 15 antibody
    • G alpha 16 antibody
    • G alpha-15 antibody
    • G alpha-16 antibody
    • G-protein subunit alpha-15 antibody
    • G-protein subunit alpha-16 antibody
    • GNA 15 antibody
    • GNA 16 antibody
    • GNA15 antibody
    • GNA15_HUMAN antibody
    • GNA16 antibody
    • Gq class antibody
    • Guanine nucleotide binding protein alpha 15 antibody
    • Guanine nucleotide binding protein alpha 15 subunit antibody
    • Guanine nucleotide-binding protein subunit alpha-15 antibody
    • Guanine nucleotide-binding protein subunit alpha-16 antibody
    see all

Images

  • Anti-G protein alpha 16 antibody (ab154157) at 1/1000 dilution + THP1 whole cell lysate at 30 µg

    Predicted band size : 44 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling G protein alpha 16 with ab154157 at 1/500 dilution. The image in the lower panel was co-stained with Hoechst 33342.

References

ab154157 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 28°C
Sample
Human Cell (G alpha16 transfected CHO-K1 cell)
Specification
G alpha16 transfected CHO-K1 cell
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 25 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (SDS-acrylamide 10%))
Sample
Human Cell lysate - whole cell (G alpha16 transfected CHO-K1 cell)
Specification
G alpha16 transfected CHO-K1 cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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