Anti-G protein alpha inhibitor 1 antibody (ab81447)

Overview

  • Product nameAnti-G protein alpha inhibitor 1 antibody
    See all G protein alpha inhibitor 1 primary antibodies
  • Description
    Rabbit polyclonal to G protein alpha inhibitor 1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 144-193, REYQLNDSAA YYLNDLDRIA QPNYIPTQQD VLRTRVKTTG IVETHFTFKD, of Human G protein alpha inhibitor 1 (NP_002060).

  • Positive control
    • Fetal Brain Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:12500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
  • Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenylate cyclase inhibiting G alpha protein antibody
    • Adenylate cyclase inhibitory protein antibody
    • Adenylate cyclase-inhibiting G alpha protein antibody
    • G protein alpha inhibiting 1 antibody
    • G protein alpha inhibiting activity polypeptide 1 antibody
    • Gi antibody
    • Gi inhibitory G protein antibody
    • Gi1 protein alpha subunit antibody
    • GNAI 1 antibody
    • GNAI1 antibody
    • GNAI1_HUMAN antibody
    • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1 antibody
    • Guanine nucleotide binding protein alpha inhibiting activity polypeptide 1 antibody
    • Guanine nucleotide binding protein G(i) alpha 1 subunit antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-1 antibody
    • OTTHUMP00000161319 antibody
    • OTTHUMP00000208047 antibody
    see all

Anti-G protein alpha inhibitor 1 antibody images

  • Anti-G protein alpha inhibitor 1 antibody (ab81447) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Anti-G protein alpha inhibitor 1 antibody (ab81447) at 1/1000 dilution + Nthy-ori cell lysate at 50 µg

    Secondary
    HRP anti-rabbit at 1/2000 dilution

    Predicted band size : 40 kDa

References for Anti-G protein alpha inhibitor 1 antibody (ab81447)

ab81447 has not yet been referenced specifically in any publications.

Product Wall

Merci pour votre émail et votre patience.
Veuillez trouver les offres de test ci-dessous.
Comme convenu, j'ai aussi fait les inquêtes pour le potentiel de crossréaction de certains anticorps contre GNAI1:
-Pour le ab81447 le laboratoire ...

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Merci de votre appel et votre intérêt pour nos anticorps contre la G protein alpha inhibitor 1 et 2.
Comme discuté au téléphone, j'ai contacté maintenant les laboratoires produisants le ab81447 et ab102014 afin d'avoir plus d'information sur la po...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"