• Product name
    Anti-G Protein alpha Inhibitor 1+2 antibody
  • Description
    Rabbit polyclonal to G Protein alpha Inhibitor 1+2
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish
  • Immunogen

    Synthetic peptide corresponding to Human G Protein alpha Inhibitor 1+2 aa 346-355.


    (Peptide available as ab5006)


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa.Can be blocked with G Protein alpha Inhibitor 1+2 peptide (ab5006).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 1 µg/ml.


Anti-G Protein alpha Inhibitor 1+2 antibody images

  • IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-G Protein alpha Inhibitor 1+2 antibody (ab3522)

This product has been referenced in:
  • Doi M  et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). WB . Read more (PubMed: 26882873) »
  • Ufer C & Germack R Cross-regulation between beta1- and beta3-adrenoceptors following chronic beta-adrenergic stimulation in neonatal rat cardiomyocytes. Br J Pharmacol 158:300-13 (2009). WB ; Rat . Read more (PubMed: 19719783) »

See all 2 Publications for this product

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