Anti-G protein alpha Inhibitor 2 antibody (ab20392)

Overview

  • Product name
    Anti-G protein alpha Inhibitor 2 antibody
    See all G protein alpha Inhibitor 2 primary antibodies
  • Description
    Rabbit polyclonal to G protein alpha Inhibitor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to Human G protein alpha Inhibitor 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab20392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
  • Sequence similarities
    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenylate cyclase inhibiting G alpha protein antibody
    • Adenylate cyclase-inhibiting G alpha protein antibody
    • GIP antibody
    • GNAI 2 antibody
    • Gnai2 antibody
    • GNAI2_HUMAN antibody
    • GNAI2B antibody
    • GTP binding regulatory protein Gi alpha 2 chain antibody
    • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
    • H LUCA15.1 antibody
    • H LUCA16.1 antibody
    • WUGSC:H LUCA15.1 antibody
    • WUGSC:H LUCA16.1 antibody
    see all

Images

  • Lanes 1 & 3 : Anti-G protein alpha Inhibitor 2 antibody (ab20392) at 1/1000 dilution
    Lane 2 : No primary

    All lanes : Rat RH7777 whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Lane 1 : No secondary
    Lanes 2-3 : Alkaline phosphatase conjugated goat anti-rabbit

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 10 minutes

    See Abreview

References

This product has been referenced in:
  • Ramírez VT  et al. Wnt-5a/Frizzled9 Receptor Signaling through the Gao-Gß? Complex Regulates Dendritic Spine Formation. J Biol Chem 291:19092-107 (2016). Read more (PubMed: 27402827) »
  • Fu X  et al. MicroRNA-222-3p/GNAI2/AKT axis inhibits epithelial ovarian cancer cell growth and associates with good overall survival. Oncotarget 7:80633-80654 (2016). WB ; Human . Read more (PubMed: 27811362) »

See all 4 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (RH7777 Cells)
Loading amount
15 µg
Specification
RH7777 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Jul 31 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up