Anti-G protein beta subunit like antibody (ab118909)

Overview

  • Product nameAnti-G protein beta subunit like antibody
    See all G protein beta subunit like primary antibodies
  • Description
    Rabbit polyclonal to G protein beta subunit like
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Monkey, Macaque Monkey
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human G protein beta subunit like.

  • Positive control
    • Human Skin tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionSubunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificityBroadly expressed, with highest levels in skeletal muscle, heart and kidney.
  • Sequence similaritiesBelongs to the WD repeat LST8 family.
    Contains 7 WD repeats.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein beta subunit like antibody
    • G protein beta subunit-like antibody
    • Gable antibody
    • GbetaL antibody
    • GBL antibody
    • GBL protein antibody
    • LST8 antibody
    • LST8_HUMAN antibody
    • Mammalian lethal with SEC13 protein 8 antibody
    • MGC111011 antibody
    • mLST8 antibody
    • MTOR associated protein LST8 homolog (S. cerevisiae) antibody
    • POP3 antibody
    • POP3 homolog (S. pombe) antibody
    • Protein GbetaL antibody
    • Target of rapamycin complex subunit LST8 antibody
    • TORC subunit LST8 antibody
    • WAT1 antibody
    • WAT1 homolog (S. pombe) antibody
    see all

Anti-G protein beta subunit like antibody images

  • Human Skin (formalin-fixed, paraffin-embedded) stained with ab118909 at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-G protein beta subunit like antibody (ab118909)

ab118909 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"