Anti-G protein beta subunit like antibody (ab25974)

Overview

  • Product nameAnti-G protein beta subunit like antibody
    See all G protein beta subunit like primary antibodies
  • Description
    Rabbit polyclonal to G protein beta subunit like
  • Specificityab25974 recognises GßL (G protein beta protein subunit-like).
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to 14 C terminal amino acids of Human G protein beta subunit like

  • Positive control
    • Human brain cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab25974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like peptide (ab39766).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
ELISA Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSubunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
    • Tissue specificityBroadly expressed, with highest levels in skeletal muscle, heart and kidney.
    • Sequence similaritiesBelongs to the WD repeat LST8 family.
      Contains 7 WD repeats.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • G protein beta subunit like antibody
      • G protein beta subunit-like antibody
      • Gable antibody
      • GbetaL antibody
      • GBL antibody
      • GBL protein antibody
      • LST8 antibody
      • LST8_HUMAN antibody
      • Mammalian lethal with SEC13 protein 8 antibody
      • MGC111011 antibody
      • mLST8 antibody
      • MTOR associated protein LST8 homolog (S. cerevisiae) antibody
      • POP3 antibody
      • POP3 homolog (S. pombe) antibody
      • Protein GbetaL antibody
      • Target of rapamycin complex subunit LST8 antibody
      • TORC subunit LST8 antibody
      • WAT1 antibody
      • WAT1 homolog (S. pombe) antibody
      see all

    Anti-G protein beta subunit like antibody images

    • Lane 1 : Anti-G protein beta subunit like antibody (ab25974) at 1 µg/ml
      Lane 2 : Anti-G protein beta subunit like antibody (ab25974) at 2 µg/ml

      Lane 1 : Human brain cell lysate
      Lane 2 : Human brain cell lysate
    • ab25974 at 10µg/ml staining G protein beta subunit like in mouse brain tissue by IHC

    • Immunofluorescence of GBL in Mouse Brain cells using ab25974 at 10 ug/ml.

    References for Anti-G protein beta subunit like antibody (ab25974)

    ab25974 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and patience. I contacted the originator of this antibody and they said that they consistently see a band at 17-20 kDa. They don't exactly know why G protein beta subunit like is running at this size, but peptide blocking exp...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Immuno-precipitation step Protein A
    Username

    Dr. Stephen Dann

    Verified customer

    Submitted Dec 20 2005

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Username

    Dr. Stephen Dann

    Verified customer

    Submitted Dec 20 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"