Anti-G-protein coupled receptor 30 antibody (ab118512)

Overview

  • Product nameAnti-G-protein coupled receptor 30 antibody
    See all G-protein coupled receptor 30 primary antibodies
  • Description
    Goat polyclonal to G-protein coupled receptor 30
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Baboon
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: C-KAVIPDSTEQSDVR, corresponding to amino acids 357-370 of Human GPCR GPR30 (NP_001496.1).

  • Positive control
    • Human adrenal and colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab118512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 - 7.5 µg/ml.

Target

  • FunctionReceptor for estrogen.
  • Tissue specificityUbiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPR antibody
    • Chemoattractant receptor-like 2 antibody
    • Chemokine receptor-like 2 antibody
    • CMKRL2 antibody
    • Constitutively expressed peptide like receptor antibody
    • DRY12 antibody
    • FEG 1 antibody
    • FEG-1 antibody
    • Flow-induced endothelial G protein-coupled receptor antibody
    • Flow-induced endothelial G-protein coupled receptor 1 antibody
    • G protein-coupled receptor 30 antibody
    • G-protein coupled estrogen receptor 1 antibody
    • G-protein coupled receptor 30 antibody
    • GPCR-BR antibody
    • Gper antibody
    • GPER_HUMAN antibody
    • GPER1 antibody
    • GPR30 antibody
    • IL8-related receptor DRY12 antibody
    • Lergu antibody
    • LERGU2 antibody
    • leucine rich protein in GPR30 3'UTR antibody
    • LYGPR antibody
    • Lymphocyte-derived G-protein coupled receptor antibody
    • Membrane estrogen receptor antibody
    • mER antibody
    • MGC99678 antibody
    see all

Anti-G-protein coupled receptor 30 antibody images

  • ab118512 at 3.75 µg/ml staining GPCR GPR30 in Formalin-Fixed, Paraffin-Embedded Human Adrenal tissue.
  • ab118512 at 3.75 µg/ml staining GPCR-GPR30 in Formalin-Fixed, Paraffin-Embedded Human Colon tissue.
  • ab118512 staining G-protein coupled receptor 30 (green) in Baboon placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (7.5µg/ml in PBS + 5% normal horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody. Red - propidium iodide-stained nuclei.

    See Abreview

References for Anti-G-protein coupled receptor 30 antibody (ab118512)

ab118512 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0
Sample Baboon Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde
Username

Ms. Marcia Burch

Verified customer

Submitted Aug 13 2014

Thank you for contacting us.  We currently have three antibodies that recognize GPCR GPR30: ab39742 - rabbit polyclonal; reacts with: human, mouse, and rat; tested for IHC-Fr and WB ab118512 - goat polyclonal; reacts with human and predicted to react w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"