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Synthetic peptide: C-KAVIPDSTEQSDVR, corresponding to amino acids 357-370 of Human GPCR GPR30 (NP_001496.1).
Our Abpromise guarantee covers the use of ab118512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3.75 - 7.5 µg/ml.|
ab118512 staining G-protein coupled receptor 30 (green) in Baboon placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (7.5µg/ml in PBS + 5% normal horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody. Red - propidium iodide-stained nuclei.
ab118512 has not yet been referenced specifically in any publications.