Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069)
Key features and details
- Rabbit polyclonal to G-protein coupled receptor 30 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-G-protein coupled receptor 30 antibody - C-terminal
See all G-protein coupled receptor 30 primary antibodies -
Description
Rabbit polyclonal to G-protein coupled receptor 30 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 307-375 of Human G-protein coupled receptor 30 (Uniprot: Q99527).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and RL952 whole cell lysates; Human endometrial tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for estrogen. -
Tissue specificity
Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor. - Information by UniProt
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Database links
- Entrez Gene: 2852 Human
- Omim: 601805 Human
- SwissProt: Q99527 Human
- Unigene: 20961 Human
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Alternative names
- CEPR antibody
- Chemoattractant receptor-like 2 antibody
- Chemokine receptor-like 2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.
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All lanes : Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS PAGE -
All lanes : Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/10000 dilution
Lane 1 : RL952 whole cell lysate
Lane 2 : RL952 whole cell lysate; transfected with G-protein coupled receptor 30 shRNA
Lysates/proteins at 40 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab154069 has been referenced in 12 publications.
- Bühler M et al. GPER1 links estrogens to centrosome amplification and chromosomal instability in human colon cells. Life Sci Alliance 6:N/A (2023). PubMed: 36384894
- Abancens M et al. GPER Agonist G1 Prevents Wnt-Induced JUN Upregulation in HT29 Colorectal Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36293473
- Zhang R et al. GPR30 knockdown weakens the capacity of CAF in promoting prostate cancer cell invasion via reducing macrophage infiltration and M2 polarization. J Cell Biochem N/A:N/A (2021). PubMed: 33938030
- Rice A et al. GPER Activation Inhibits Cancer Cell Mechanotransduction and Basement Membrane Invasion via RhoA. Cancers (Basel) 12:N/A (2020). PubMed: 31991740
- Cortes E et al. Tamoxifen mechanically deactivates hepatic stellate cells via the G protein-coupled estrogen receptor. Oncogene 38:2910-2922 (2019). PubMed: 30575816