Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069)

Overview

  • Product name
    Anti-G-protein coupled receptor 30 antibody - C-terminal
    See all G-protein coupled receptor 30 primary antibodies
  • Description
    Rabbit polyclonal to G-protein coupled receptor 30 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 307-375 of Human G-protein coupled receptor 30 (Uniprot: Q99527).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and RL952 whole cell lysates; Human endometrial tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Receptor for estrogen.
  • Tissue specificity
    Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPR antibody
    • Chemoattractant receptor-like 2 antibody
    • Chemokine receptor-like 2 antibody
    • CMKRL2 antibody
    • Constitutively expressed peptide like receptor antibody
    • DRY12 antibody
    • FEG 1 antibody
    • FEG-1 antibody
    • Flow-induced endothelial G protein-coupled receptor antibody
    • Flow-induced endothelial G-protein coupled receptor 1 antibody
    • G protein-coupled receptor 30 antibody
    • G-protein coupled estrogen receptor 1 antibody
    • G-protein coupled receptor 30 antibody
    • GPCR-BR antibody
    • Gper antibody
    • GPER_HUMAN antibody
    • GPER1 antibody
    • GPR30 antibody
    • IL8-related receptor DRY12 antibody
    • Lergu antibody
    • LERGU2 antibody
    • leucine rich protein in GPR30 3'UTR antibody
    • LYGPR antibody
    • Lymphocyte-derived G-protein coupled receptor antibody
    • Membrane estrogen receptor antibody
    • mER antibody
    • MGC99678 antibody
    see all

Images

  • All lanes : Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
  • All lanes : Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/10000 dilution

    Lane 1 : RL952 whole cell lysate
    Lane 2 : RL952 whole cell lysate; transfected with G-protein coupled receptor 30 shRNA

    Lysates/proteins at 40 µg per lane.


    Predicted band size : 42 kDa
  • Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.

References

This product has been referenced in:
  • Zhou L  et al. G-protein-coupled receptor 30 mediates the effects of estrogen on endothelial cell tube formation in vitro. Int J Mol Med 39:1461-1467 (2017). WB, ICC/IF ; Human . Read more (PubMed: 28440394) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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