Overview

  • Product name
    Anti-G0 Protein alpha antibody
    See all G0 Protein alpha primary antibodies
  • Description
    Rabbit polyclonal to G0 Protein alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 104-338 of Human G0 Protein alpha.

  • Positive control
    • A549, HeLa, Jurkat, NT2D1 and mouse brain lysates; HeLa cells; Human lung adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-G0 Protein alpha antibody (ab154001) at 1/10000 dilution + Mouse brain whole cell
    at 5 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • All lanes : Anti-G0 Protein alpha antibody (ab154001) at 1/1000 dilution

    Lane 1 : HeLa whole cell
    lysate
    Lane 2 : NT2D1 whole cell
    lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling G0 Protein alpha with ab154001 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling G0 Protein alpha with ab154001 at 1/500 dilution.

References

ab154001 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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