• Product nameAnti-G3BP2 antibody
    See all G3BP2 primary antibodies
  • Description
    Rabbit polyclonal to G3BP2
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429.2)

  • Positive control
    • Whole cell lysate from HeLa cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab86135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.


  • FunctionProbable scaffold protein that may be involved in mRNA transport.
  • Sequence similaritiesContains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • G3BP 2 antibody
    • G3BP-2 antibody
    • G3BP2 antibody
    • G3BP2_HUMAN antibody
    • GAP SH3 domain binding protein 2 antibody
    • GAP SH3 domain-binding protein 2 antibody
    • GTPase activating protein (SH3 domain) binding protein 2 antibody
    • Ras GTPase activating protein SH3 domain binding protein 2 antibody
    • Ras GTPase-activating protein-binding protein 2 antibody
    see all

Anti-G3BP2 antibody images

  • All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    developed using the ECL technique

    Predicted band size : 54 kDa

    Exposure time : 60 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
    20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-G3BP2 antibody (ab86135)

This product has been referenced in:
  • Zhang H  et al. Downregulation of G3BPs inhibits the growth, migration and invasion of human lung carcinoma H1299 cells by suppressing the Src/FAK-associated signaling pathway. Cancer Gene Ther 20:622-9 (2013). Human . Read more (PubMed: 24157923) »
  • Culver BP  et al. Proteomic analysis of wild-type and mutant huntingtin associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis. J Biol Chem : (2012). Read more (PubMed: 22556411) »

See all 2 Publications for this product

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