• Product name
  • Description
    Rabbit polyclonal to G3BP2
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429.2)

  • Positive control
    • Whole cell lysate from HeLa cells


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab86135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function
    Probable scaffold protein that may be involved in mRNA transport.
  • Sequence similarities
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • G3BP 2 antibody
    • G3BP-2 antibody
    • G3BP2 antibody
    • G3BP2_HUMAN antibody
    • GAP SH3 domain binding protein 2 antibody
    • GAP SH3 domain-binding protein 2 antibody
    • GTPase activating protein (SH3 domain) binding protein 2 antibody
    • Ras GTPase activating protein SH3 domain binding protein 2 antibody
    • Ras GTPase-activating protein-binding protein 2 antibody
    see all


  • All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa

    Exposure time : 60 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
    20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Kim DY  et al. New World and Old World Alphaviruses Have Evolved to Exploit Different Components of Stress Granules, FXR and G3BP Proteins, for Assembly of Viral Replication Complexes. PLoS Pathog 12:e1005810 (2016). Read more (PubMed: 27509095) »
  • Viktorovskaya OV  et al. Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements. PLoS Negl Trop Dis 10:e0004921 (2016). Read more (PubMed: 27556644) »

See all 4 Publications for this product

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