GTPase activating protein (SH3 domain) binding protein 2 antibody
Ras GTPase activating protein SH3 domain binding protein 2 antibody
Ras GTPase-activating protein-binding protein 2 antibody
Western blot - G3BP2 antibody (ab86135)
All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 60 seconds
Immunoprecipitation - G3BP2 antibody (ab86135)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Gupta N et al. Stress granule-associated protein G3BP2 regulates breast tumor initiation. Proc Natl Acad Sci U S A114:1033-1038 (2017).
Read more (PubMed: 28096337) »
Kim DY et al. New World and Old World Alphaviruses Have Evolved to Exploit Different Components of Stress Granules, FXR and G3BP Proteins, for Assembly of Viral Replication Complexes. PLoS Pathog12:e1005810 (2016).
Read more (PubMed: 27509095) »