GTPase activating protein (SH3 domain) binding protein 2 antibody
Ras GTPase activating protein SH3 domain binding protein 2 antibody
Ras GTPase-activating protein-binding protein 2 antibody
Anti-G3BP2 antibody images
Western blot - G3BP2 antibody (ab86135)
All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg
developed using the ECL technique
Predicted band size : 54 kDa
Exposure time : 60 seconds
Immunoprecipitation - G3BP2 antibody (ab86135)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-G3BP2 antibody (ab86135)
This product has been referenced in:
Zhang H et al. Downregulation of G3BPs inhibits the growth, migration and invasion of human lung carcinoma H1299 cells by suppressing the Src/FAK-associated signaling pathway. Cancer Gene Ther20:622-9 (2013).
Read more (PubMed: 24157923) »
Culver BP et al. Proteomic analysis of wild-type and mutant huntingtin associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis. J Biol Chem : (2012).
Read more (PubMed: 22556411) »