Overview

  • Product nameAnti-G3BP2 antibody
    See all G3BP2 primary antibodies
  • Description
    Rabbit polyclonal to G3BP2
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429.2)

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab86135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionProbable scaffold protein that may be involved in mRNA transport.
  • Sequence similaritiesContains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • G3BP 2 antibody
    • G3BP-2 antibody
    • G3BP2 antibody
    • G3BP2_HUMAN antibody
    • GAP SH3 domain binding protein 2 antibody
    • GAP SH3 domain-binding protein 2 antibody
    • GTPase activating protein (SH3 domain) binding protein 2 antibody
    • Ras GTPase activating protein SH3 domain binding protein 2 antibody
    • Ras GTPase-activating protein-binding protein 2 antibody
    see all

Anti-G3BP2 antibody images

  • All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 60 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
    20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-G3BP2 antibody (ab86135)

This product has been referenced in:
  • Kim DY  et al. New World and Old World Alphaviruses Have Evolved to Exploit Different Components of Stress Granules, FXR and G3BP Proteins, for Assembly of Viral Replication Complexes. PLoS Pathog 12:e1005810 (2016). Read more (PubMed: 27509095) »
  • Zhang H  et al. Downregulation of G3BPs inhibits the growth, migration and invasion of human lung carcinoma H1299 cells by suppressing the Src/FAK-associated signaling pathway. Cancer Gene Ther 20:622-9 (2013). Human . Read more (PubMed: 24157923) »

See all 3 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"