Overview

  • Product nameAnti-GAB1 antibody
    See all GAB1 primary antibodies
  • Description
    Rabbit polyclonal to GAB1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 644-694 of Human GAB1 (NP_002030.2).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab117774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionProbably involved in EGF and insulin receptor signaling.
  • Sequence similaritiesBelongs to the GAB family.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Anti-GAB1 antibody images

  • All lanes : Anti-GAB1 antibody (ab117774) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa


    Exposure time : 3 minutes
  • Detection of GAB1 by Western Blot of Immunprecipitate.
    ab117774 at 1µg/ml staining GAB1 in HeLa whole cell lysate immunoprecipitated using ab117774 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-GAB1 antibody (ab117774)

ab117774 has not yet been referenced specifically in any publications.

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