Overview

  • Product nameAnti-GAB1 antibody
    See all GAB1 primary antibodies
  • Description
    Rabbit polyclonal to GAB1
  • Specificityab59362 detects endogenous levels of total GAB1 protein
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized non phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).

  • Positive control
    • NIH/3T3 cytoplasmic lysate (WB) Human breast carcinoma tissue (IHC)
  • General notesStore at -20°C for 1 year

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionProbably involved in EGF and insulin receptor signaling.
  • Sequence similaritiesBelongs to the GAB family.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Anti-GAB1 antibody images

  • All lanes : Anti-GAB1 antibody (ab59362) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
    Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide


    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
  • ab59362, at a 1/50 dilution, staining human GAB1 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
  • ICC/IF image of ab59362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59362, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GAB1 antibody (ab59362)

ab59362 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for contacting us. The product was tested on  NIH/3T3 cells lysate. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"