Overview

  • Product name
    Anti-GAB1 antibody [Y423]
    See all GAB1 primary antibodies
  • Description
    Rabbit monoclonal [Y423] to GAB1
  • Specificity
    This antibody is specific for the splice isoform 2 of human GAB1.
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GAB1.

  • Positive control
    • WB: 293 cell lysate. ICC/IF: PC-12 cells. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-GAB1 antibody (Alexa Fluor® 488) [Y423] (ab208232)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
ICC/IF 1/50.
Flow Cyt 1/2500.
  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Function
      Probably involved in EGF and insulin receptor signaling.
    • Sequence similarities
      Belongs to the GAB family.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gab 1 antibody
      • GAB1 antibody
      • GAB1_HUMAN antibody
      • GRB 2 associated binder 1 antibody
      • GRB 2 associated binding protein 1 antibody
      • GRB2 associated binding protein 1 isoform a antibody
      • GRB2 associated binding protein 1 isoform b antibody
      • GRB2-associated binder 1 antibody
      • GRB2-associated-binding protein 1 antibody
      • Growth factor receptor bound protein 2-associated protein 1 antibody
      see all

    Anti-GAB1 antibody [Y423] images

    • Anti-GAB1 antibody [Y423] (ab32093) at 1/5000 dilution + 293 cell lysate.

      Predicted band size : 76 kDa
      Observed band size : 76 kDa
    • ab32093 at a 1:50 dilution staining GAB1 in PC-12 cells using immunofluoresence
    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GAB1 (red) with ab32093 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    References for Anti-GAB1 antibody [Y423] (ab32093)

    This product has been referenced in:
    • Le Goff A  et al. Anti-apoptotic Role of Caspase-cleaved GAB1 Adaptor Protein in Hepatocyte Growth Factor/Scatter Factor-MET Receptor Protein Signaling. J Biol Chem 287:35382-96 (2012). WB . Read more (PubMed: 22915589) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab32093.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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