Anti-GAB1 (phospho Y627) antibody (ab47771)

Overview

  • Product nameAnti-GAB1 (phospho Y627) antibody
    See all GAB1 primary antibodies
  • Description
    Rabbit polyclonal to GAB1 (phospho Y627)
  • SpecificityThis antibody detects Gab1 only when phosphorylated at tyrosine 627.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D).

  • Positive control
    • HuvEc cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab47771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 77 kDa).
ELISA 1/10000.

Target

  • FunctionProbably involved in EGF and insulin receptor signaling.
  • Sequence similaritiesBelongs to the GAB family.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Anti-GAB1 (phospho Y627) antibody images

  • All lanes : Anti-GAB1 (phospho Y627) antibody (ab47771) at 1/500 dilution

    Lane 1 : HuvEc cell extract, untreated.
    Lane 2 : HuvEc cell extract treated with EGF (200ng/ml, 10min)


    Predicted band size : 77 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

References for Anti-GAB1 (phospho Y627) antibody (ab47771)

ab47771 has not yet been referenced specifically in any publications.

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