Anti-GAB1 (phospho Y659) antibody (ab59410)

Overview

  • Product nameAnti-GAB1 (phospho Y659) antibody
    See all GAB1 primary antibodies
  • Description
    Rabbit polyclonal to GAB1 (phospho Y659)
  • SpecificityThis antibody detects endogenous levels of GAB1 only when phosphorylated at tyrosine 659. Human: Tyr659; Mouse: Tyr660.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).

  • Positive control
    • NIH/3T3 cell extracts

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/5000.

Target

  • FunctionProbably involved in EGF and insulin receptor signaling.
  • Sequence similaritiesBelongs to the GAB family.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Anti-GAB1 (phospho Y659) antibody images

  • All lanes : Anti-GAB1 (phospho Y659) antibody (ab59410) at 1/500 dilution

    Lane 1 : NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins)
    Lane 2 : NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins) with immunizing phosphopeptide


    Predicted band size : 77 kDa
    Observed band size : 77 kDa

References for Anti-GAB1 (phospho Y659) antibody (ab59410)

ab59410 has not yet been referenced specifically in any publications.

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