Overview

  • Product nameAnti-GAB2 antibody [EP369Y]
    See all GAB2 primary antibodies
  • Description
    Rabbit monoclonal [EP369Y] to GAB2
  • SpecificityThis antibody is predicted to detect the splice isoform 2 based on sequence homology.
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in human Gab2

  • Positive control
    • PC12 cells; K562 cells; MCF7 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
ICC/IF 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IHC or IP.
  • Target

    • FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
    • Sequence similaritiesBelongs to the GAB family.
      Contains 1 PH domain.
    • DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
      The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
      Dephosphorylated by PTPN11.
    • Cellular localizationCytoplasm. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GAB 2 antibody
      • Gab2 antibody
      • GAB2_HUMAN antibody
      • Grb 2 associated binder 2 antibody
      • GRB 2 associated binding protein 2 antibody
      • Grb2 associated binder 2 antibody
      • GRB2 associated binder 2 pp100 antibody
      • GRB2 associated binding protein 2 antibody
      • GRB2-associated binder 2 antibody
      • GRB2-associated-binding protein 2 antibody
      • Growth factor receptor bound protein 2 associated protein 2 antibody
      • Growth factor receptor bound protein 2-associated protein 2 antibody
      • KIAA0571 antibody
      • p97 antibody
      • PH domain containing adaptor molecule p97 antibody
      • pp100 antibody
      see all

    Anti-GAB2 antibody [EP369Y] images

    • All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution

      Lane 1 : K526 cell lysate
      Lane 2 : MCF-7 cell lysate


      Predicted band size : 74 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of PC-12 cells
      using ab32365 at 1/50 dilution

    References for Anti-GAB2 antibody [EP369Y] (ab32365)

    This product has been referenced in:
    • Tian LQ  et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . Read more (PubMed: 27035789) »
    • Pyarajan S  et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . Read more (PubMed: 18586679) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    In order to ...

    Read More

    Hello! The blocking peptides for these antibodies have now been published in our catalog, they are ab133951 and ab133952. Please let me know if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"