Anti-GAB2 (phospho S623) antibody (ab92578)


  • Product nameAnti-GAB2 (phospho S623) antibody
    See all GAB2 primary antibodies
  • Description
    Rabbit polyclonal to GAB2 (phospho S623)
  • SpecificityDetects endogenous levels of Gab2 only when phosphorylated at Serine 623.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human GAB2 around the phosphorylation site of Serine 623 (PS-SP-P-S).

  • Positive control
    • WB: lysate from HepG2 cells treated with PMA (125ng/ml, 30mins). IHC-P: Human breast carcinoma tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab92578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/10,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 74 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
    • Sequence similaritiesBelongs to the GAB family.
      Contains 1 PH domain.
    • DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
      The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
    • Post-translational
      Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
      Dephosphorylated by PTPN11.
    • Cellular localizationCytoplasm. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GAB 2 antibody
      • Gab2 antibody
      • GAB2_HUMAN antibody
      • Grb 2 associated binder 2 antibody
      • GRB 2 associated binding protein 2 antibody
      • Grb2 associated binder 2 antibody
      • GRB2 associated binder 2 pp100 antibody
      • GRB2 associated binding protein 2 antibody
      • GRB2-associated binder 2 antibody
      • GRB2-associated-binding protein 2 antibody
      • Growth factor receptor bound protein 2 associated protein 2 antibody
      • Growth factor receptor bound protein 2-associated protein 2 antibody
      • KIAA0571 antibody
      • p97 antibody
      • PH domain containing adaptor molecule p97 antibody
      • pp100 antibody
      see all

    Anti-GAB2 (phospho S623) antibody images

    • All lanes : Anti-GAB2 (phospho S623) antibody (ab92578) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells treated with PMA (125ng/ml, 30mins)
      Lane 2 : Extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins) with immunizing phosphopeptide at 10 µg

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 74 kDa
    • Left panel: ab92578, at a 1/50 dilution, staining phosphorylated Gab2 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue.
      Right panel: same, after pre-incubation with the immunizing phosphopeptide.

    References for Anti-GAB2 (phospho S623) antibody (ab92578)

    ab92578 has not yet been referenced specifically in any publications.

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