Overview

  • Product name
    Anti-GAB2 (phospho Y643) antibody
    See all GAB2 primary antibodies
  • Description
    Rabbit polyclonal to GAB2 (phospho Y643)
  • Specificity
    This antibody detects endogenous levels of GAB2 only when phosphorylated at tyrosine 643.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of tyrosine 643 (V-D-YP-V-Q).

  • Positive control
    • Extracts from Jurkat cells. Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • Function
    Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
  • Sequence similarities
    Belongs to the GAB family.
    Contains 1 PH domain.
  • Domain
    The SH3-binding motifs mediate interaction with SHC1 and GRB2.
    The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
    Dephosphorylated by PTPN11.
  • Cellular localization
    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAB 2 antibody
    • Gab2 antibody
    • GAB2_HUMAN antibody
    • Grb 2 associated binder 2 antibody
    • GRB 2 associated binding protein 2 antibody
    • Grb2 associated binder 2 antibody
    • GRB2 associated binder 2 pp100 antibody
    • GRB2 associated binding protein 2 antibody
    • GRB2-associated binder 2 antibody
    • GRB2-associated-binding protein 2 antibody
    • Growth factor receptor bound protein 2 associated protein 2 antibody
    • Growth factor receptor bound protein 2-associated protein 2 antibody
    • KIAA0571 antibody
    • p97 antibody
    • PH domain containing adaptor molecule p97 antibody
    • pp100 antibody
    see all

Images

  • All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins)
    Lane 2 : Extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References

This product has been referenced in:
  • Yim JH  et al. Phosphoprotein profiles of candidate markers for early cellular responses to low-dose ?-radiation in normal human fibroblast cells. J Radiat Res 58:329-340 (2017). WB ; Human . Read more (PubMed: 28122968) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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