Anti-GAB2 (phospho Y643) antibody (ab78262)

Overview

  • Product nameAnti-GAB2 (phospho Y643) antibody
    See all GAB2 primary antibodies
  • Description
    Rabbit polyclonal to GAB2 (phospho Y643)
  • SpecificityThis antibody detects endogenous levels of GAB2 only when phosphorylated at tyrosine 643.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of tyrosine 643 (V-D-YP-V-Q).

  • Positive control
    • Extracts from Jurkat cells. Human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
  • Sequence similaritiesBelongs to the GAB family.
    Contains 1 PH domain.
  • DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
    The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
    Dephosphorylated by PTPN11.
  • Cellular localizationCytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAB 2 antibody
    • Gab2 antibody
    • GAB2_HUMAN antibody
    • Grb 2 associated binder 2 antibody
    • GRB 2 associated binding protein 2 antibody
    • Grb2 associated binder 2 antibody
    • GRB2 associated binder 2 pp100 antibody
    • GRB2 associated binding protein 2 antibody
    • GRB2-associated binder 2 antibody
    • GRB2-associated-binding protein 2 antibody
    • Growth factor receptor bound protein 2 associated protein 2 antibody
    • Growth factor receptor bound protein 2-associated protein 2 antibody
    • KIAA0571 antibody
    • p97 antibody
    • PH domain containing adaptor molecule p97 antibody
    • pp100 antibody
    see all

Anti-GAB2 (phospho Y643) antibody images

  • All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins)
    Lane 2 : Extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References for Anti-GAB2 (phospho Y643) antibody (ab78262)

ab78262 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"