Overview

  • Product nameAnti-GAB3 antibody
    See all GAB3 primary antibodies
  • Description
    Rabbit polyclonal to GAB3
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 336-480 of Human GAB3.

  • Positive control
    • Human Plasma, Liver and Tonsil lysates; Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab121311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similaritiesBelongs to the GAB family.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gab3 antibody
    • GAB3_HUMAN antibody
    • GRB2-associated binder 3 antibody
    • GRB2-associated-binding protein 3 antibody
    • Growth factor receptor bound protein 2-associated protein 3 antibody
    see all

Anti-GAB3 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.

References for Anti-GAB3 antibody (ab121311)

ab121311 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121311.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"