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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
- Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
modificationsPhosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
Information by UniProt
- Gab3 antibody
- GAB3_HUMAN antibody
- GRB2-associated binder 3 antibody
Anti-GAB3 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-GAB3 antibody (ab121311)
All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)
ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
References for Anti-GAB3 antibody (ab121311)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"