Overview

  • Product name
  • Description
    Rabbit polyclonal to GAB4
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ESTGFLGNIS SASHGLCSSP AEPSCSHQHL PQEQEPTSEP PVSHCVPPTW PIPAPPGCLR SHQHASQRAE, corresponding to amino acids 157-226 of Human GAB4.

  • Positive control
    • Human colon tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab126474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the GAB family.
    Contains 1 PH domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAB2-like antibody
    • GAB4 antibody
    • GAB4_HUMAN antibody
    • GRB2 associated binder 2 like protein antibody
    • GRB2 associated binding protein family member 4 antibody
    • GRB2-associated binder 2-like antibody
    • GRB2-associated binder 4 antibody
    • GRB2-associated-binding protein 2-like antibody
    • GRB2-associated-binding protein 4 antibody
    • Growth factor receptor bound protein 2-associated protein 4 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab126474 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126474, at 1/200 dilution, staining GAB4 in paraffin-embedded Human colon tissue by Immunohistochemistry.

References

ab126474 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab126474.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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