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Our Abpromise guarantee covers the use of ab33299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. PubMed: 20650017|
|WB||1/10000. Predicted molecular weight: 52 kDa.|
|IP||Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.|
|IHC-Fr||Use at an assay dependent concentration. Note: use 4% para glutaraldehyde fixed frozen sections permeabilized with Triton X100.|
Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.
Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
Receptor a1 (~50kD).
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