Anti-GABA A Receptor alpha 2 antibody (ab72445)

Overview

  • Product name
    Anti-GABA A Receptor alpha 2 antibody
    See all GABA A Receptor alpha 2 primary antibodies
  • Description
    Rabbit polyclonal to GABA A Receptor alpha 2
  • Specificity
    ab72445 is specific for GABA A Receptor alpha 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Dog, Human, Non human primates, Zebrafish
  • Immunogen

    Fusion protein from the cytoplasmic loop of rat GABA A Receptor alpha 2

  • Positive control
    • rat brain lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab72445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GABA A receptor subunit alpha 2 antibody
    • GABA antibody
    • GABA(A) receptor subunit alpha 2 antibody
    • GABA(A) receptor subunit alpha-2 antibody
    • GABR A2 antibody
    • GABRA 2 antibody
    • GABRA2 antibody
    • GABRA2 protein antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
    • Gamma aminobutyric acid A receptor alpha 2 antibody
    • Gamma aminobutyric acid receptor alpha 2 subunit antibody
    • Gamma aminobutyric acid receptor subunit alpha 2 antibody
    • Gamma-aminobutyric acid receptor subunit alpha-2 antibody
    • GBRA2_HUMAN antibody
    see all

Images

  • All lanes : Anti-GABA A Receptor alpha 2 antibody (ab72445) at 1/1000 dilution

    Lane 1 : Wild type Mouse brain lysates
    Lane 2 : GABA A Receptor alpha 2 knockout mouse brain lysate


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

References

This product has been referenced in:
  • Kaneko Y  et al. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 6:35659 (2016). WB ; Rat . Read more (PubMed: 27767042) »
  • Bates RC  et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). Read more (PubMed: 24836856) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Frontal cortex)
Permeabilization
Yes - Triton X-100
Specification
Brain, Frontal cortex
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 29 2017

Thank you for contacting Abcam regarding the immunogen information for ab72445 and ab103707.

Regarding ab103707, the immunogen was generated from a peptide within aa53-83, corresponding to an extracellular domain of the protein. The specific a...

Read More

Thank you for your reply.

There are few protocol variations that may help improve the results that your are getting:

1 - Do not heat/boil your samples. When looking at transmembrane proteins, it is advisable to not heat the sample a...

Read More

Thank you for contacting Abcam.

I would be more than happy to help out in any way that I can. I just have a few more protocol questions for you:

1 - Are you boiling your samples? If so for how long?

2 - What percentage of...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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