Anti-GABA A Receptor alpha 2 antibody [N399/19] (ab193311)

Overview

  • Product name
    Anti-GABA A Receptor alpha 2 antibody [N399/19]
    See all GABA A Receptor alpha 2 primary antibodies
  • Description
    Mouse monoclonal [N399/19] to GABA A Receptor alpha 2
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat GABA A Receptor alpha 2 aa 350-450.

  • Positive control
    • ICC/IF: SKNSH cell line.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab193311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52,104 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GABA A receptor subunit alpha 2 antibody
    • GABA antibody
    • GABA(A) receptor subunit alpha 2 antibody
    • GABA(A) receptor subunit alpha-2 antibody
    • GABR A2 antibody
    • GABRA 2 antibody
    • GABRA2 antibody
    • GABRA2 protein antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
    • Gamma aminobutyric acid A receptor alpha 2 antibody
    • Gamma aminobutyric acid receptor alpha 2 subunit antibody
    • Gamma aminobutyric acid receptor subunit alpha 2 antibody
    • Gamma-aminobutyric acid receptor subunit alpha-2 antibody
    • GBRA2_HUMAN antibody
    see all

Images

  • All lanes : Anti-GABA A Receptor alpha 2 antibody [N399/19] (ab193311) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Pancreas (Rat) Tissue Lysate
    Lane 5 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 6 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Additional bands at : 104 kDa (possible dimer).

    Exposure time : 20 minutes
  • ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References

This product has been referenced in:
  • Zhang W  et al. Effect of Yi-nao-jie-yu decoction on ?-aminobutyric acid type A receptor in the hippocampus and serum inflammatory factors in a rat model of poststroke anxiety. Neuropsychiatr Dis Treat 12:2827-2837 (2016). WB ; Rat . Read more (PubMed: 27843317) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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