Western blot - Anti-GABA A Receptor alpha 2 antibody [N399/19] (ab193311)
All lanes : Anti-GABA A Receptor alpha 2 antibody [N399/19] (ab193311) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : Human brain tissue lysate - total protein (ab29466) Lane 4 : Pancreas (Rat) Tissue Lysate Lane 5 : Human pancreas tissue lysate - total protein (ab29816) Lane 6 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 51 kDa Additional bands at : 104 kDa (possible dimer).
ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Zhang W et al. Effect of Yi-nao-jie-yu decoction on ?-aminobutyric acid type A receptor in the hippocampus and serum inflammatory factors in a rat model of poststroke anxiety. Neuropsychiatr Dis Treat12:2827-2837 (2016).
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