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Rabbit polyclonal to GABA A Receptor alpha 3 - BSA and Azide free
HumanPredicted to work with:
Mouse, Rat, Cow, Cat, Dog
Synthetic peptide corresponding to a region between amino acids 20-492 of Human GABA A Receptor alpha 3
- Jurkat, HepG2, or Raji cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA3 sub-subfamily.
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
- GABA A Receptor alpha 3 antibody
- GABA(A) receptor subunit alpha 3 antibody
- GABA(A) receptor subunit alpha-3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3 antibody - BSA and Azide free (ab23334)
Western blot - GABA A Receptor alpha 3 antibody - BSA and Azide free (ab23334)
All lanes :
Anti-GABA A Receptor alpha 3 antibody - BSA and Azide free (ab23334) at 1 µg/mlLane 1 :
Jurkat Cell lysateLane 2 :
HepG2 Cell lysateLane 3 :
GABRA3 Cell lysatePredicted band size :
55 kDaObserved band size :
58 kDa (why is the actual band size different from the predicted?
HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"