Anti-GABA A Receptor alpha 4 antibody (ab94716)

Overview

  • Product nameAnti-GABA A Receptor alpha 4 antibody
    See all GABA A Receptor alpha 4 primary antibodies
  • Description
    Rabbit polyclonal to GABA A Receptor alpha 4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide to a region within: VSAKKVPAIA LSAGVSFALL RFLCLAVCLN ESPGQNQKEE KLCTENFTRI , corresponding to N terminal amino acids 2-51 of Human GABA A Receptor alpha 4 (NP_000800).

  • Positive control
    • ACHN cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GABA(A) receptor subunit alpha 4 antibody
    • GABA(A) receptor subunit alpha-4 antibody
    • GABR A4 antibody
    • GABRA 4 antibody
    • Gabra4 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 4 antibody
    • Gamma aminobutyric acid A receptor alpha 4 antibody
    • Gamma aminobutyric acid receptor alpha 4 subunit antibody
    • Gamma aminobutyric acid receptor subunit alpha 4 antibody
    • Gamma-aminobutyric acid receptor subunit alpha-4 antibody
    • GBRA4_HUMAN antibody
    see all

Anti-GABA A Receptor alpha 4 antibody images

  • Anti-GABA A Receptor alpha 4 antibody (ab94716) at 1 µg/ml (in 5% skim milk / PBS buffer) + ACHN cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa

References for Anti-GABA A Receptor alpha 4 antibody (ab94716)

ab94716 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.01% triton
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Formaldehyde
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Submitted Sep 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"