Anti-GABA A Receptor alpha 5 antibody (ab10098)

Overview

  • Product nameAnti-GABA A Receptor alpha 5 antibody
    See all GABA A Receptor alpha 5 primary antibodies
  • Description
    Rabbit polyclonal to GABA A Receptor alpha 5
  • Tested applicationsSuitable for: ELISA, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to the middle region of Mouse GABA A Receptor alpha 5 (NP_795916.1).

  • Positive control
    • Mouse muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab10098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Positive result against immunising peptide.
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 55.4 kDa).Can be blocked with GABA A Receptor alpha 5 peptide (ab137922). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-subfamily.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAA 5 antibody
    • GAA5 antibody
    • GABA(A) receptor subunit alpha-5 antibody
    • GABRA 5 antibody
    • Gabra5 antibody
    • Gamma aminobutyric acid GABA A receptor alpha 5 antibody
    • Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody
    • Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody
    • Gamma-aminobutyric acid receptor subunit alpha-5 antibody
    • GBRA5_HUMAN antibody
    • GC138184 antibody
    see all

Anti-GABA A Receptor alpha 5 antibody images

  • All lanes : Anti-GABA A Receptor alpha 5 antibody (ab10098) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse brain lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    IRDye® 680LT-conjugated donkey anti-rabbit IgG polyclonal (undiluted)

    Performed under reducing conditions.

    Predicted band size : 55.4 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)


    Exposure time : 6 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab10098 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab10098 staining GABA A Receptor alpha 5 in rat hippocampus sections by Immunohistochemistry (Frozen sections).
    Rats were transcardially perfused with 4% PFA, the brain was cryoprotected in 30% sucrose before flash freezing. Coronal sections were taken using cryostat (20 micron sections) and mounted onto slides. Tissue was fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were then blocked using 10% serum for 1 hour at 20°C and then incubated with ab10098 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at a 1/600 dilution.

    An antigen retrieval step improved results (5% SDS for 5 minutes before standard IHC protocol).

    See Abreview

  • Anti-GABA A Receptor alpha 5 antibody (ab10098) at 1 µg/ml + Mouse Liver Lysate

    Predicted band size : 55.4 kDa

References for Anti-GABA A Receptor alpha 5 antibody (ab10098)

This product has been referenced in:
  • Fatemi SH  et al. Expression of GABAA a2-, ß1- and ?-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder. Transl Psychiatry 3:e303 (2013). WB ; Human . Read more (PubMed: 24022508) »
  • Farisello P  et al. Synaptic and Extrasynaptic Origin of the Excitation/Inhibition Imbalance in the Hippocampus of Synapsin I/II/III Knockout Mice. Cereb Cortex : (2012). Mouse . Read more (PubMed: 22368083) »

See all 3 Publications for this product

Product Wall

Thank you for providing me with that information. This will be very helpful for us in trying to understand what may have contributed to the problems observed.

I would like to point out with the new antibody (ab83003). This antibody has shown ...

Read More
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mrs. Olga Cravetchi

Verified customer

Submitted Jul 12 2016

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Cell lysate - other (Brain)
Specification Brain
Blocking step LI-COR Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2015

Thank you for contacting us regarding controls to use with ab10098.

Use of a blocking peptide will determine whether non-specific binding of an antibody to proteins other than the antigen is occurring.I have been able to add the blocking pep...

Read More

Thank you for calling us today to report the problems you are continuing to have with the anti-GABA A Receptor alpha 5 antibody (ab10098). As discussed over the phone, I have arranged for a replacement vial of the antibody ab83003 to be sent to you. Th...

Read More

Thank you for contacting us and your interest in our products.
We would indeed be very interested in your customer trying anti-GABA A Receptor alpha 5 antibody (ab10098) with rat samples. By comparing the sequence of the immunogen used against the...

Read More

Thank you for contacting us.


I now have some more information to share with you about the specificity of Anti-GABA A Receptor alpha 5 antibody (ab10098). A western blot with mouse muscle tissue lysate detecting a clean band at theexpect...

Read More

Thank you for your reply.

Based on all of the information you have provided, I am wondering if the problem is due to auto fluorescence, as I cannot think of any other way that you would see background signal in your no secondary condition, as...

Read More

Thank you for your reply.

Is your negative control, the sections without any primary antibody present? I ask as I am trying to narrow down if the issue is with the primary or secondary antibody.

In the original protocol information ...

Read More

Thank you for contacting Abcam.

I would be happy to help you with some support regarding ab10098. Could you please answer the questions below about the protocol that you are using and also include the type of tissue/cells you are using and th...

Read More

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"