Anti-GABA A Receptor beta 1 (phospho S434) antibody (ab51123)

Overview

  • Product name
    Anti-GABA A Receptor beta 1 (phospho S434) antibody
    See all GABA A Receptor beta 1 primary antibodies
  • Description
    Rabbit polyclonal to GABA A Receptor beta 1 (phospho S434)
  • Specificity
    Ab51123 detects endogenous levels of GABA A Receptor beta 1 only when phosphorylated at serine 434.
  • Tested applications
    Suitable for: ELISA, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human GABA-RB around the phosphorylation site of serine 434 (R-A-SP-Q-L).

  • Positive control
    • Human breast carcinoma tissue or extracts from cos7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab51123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).

Target

  • Function
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW061132 antibody
    • B230208N19Rik antibody
    • GABA(A) receptor beta 1 antibody
    • GABA(A) receptor subunit beta-1 antibody
    • GABA-A receptor, beta-1 polypeptide antibody
    • Gabrb-1 antibody
    • GABRB1 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
    • Gamma Aminobutyric Acid A Receptor Beta 1 antibody
    • Gamma Aminobutyric Acid Receptor , beta-1 antibody
    • Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody
    • Gamma-aminobutyric acid receptor subunit beta-1 antibody
    • GARB1 antibody
    • GBRB1_HUMAN antibody
    see all

Images

  • This image shows paraffin-embedded human breast carcinoma tissue stained with ab51123 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
  • All lanes : Anti-GABA A Receptor beta 1 (phospho S434) antibody (ab51123)

    Lane 1 : Extracts from cos7 cells with immunising peptide
    Lane 2 : Extracts from cos7 cells with no peptide


    Predicted band size : 53 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab51123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Kim JH  et al. Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth. PLoS One 9:e102893 (2014). IHC-P ; Human . Read more (PubMed: 25047033) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (tooth)
Specification
tooth
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

Submitted Jul 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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