Anti-GABA A Receptor beta 3 antibody [N87/25] (ab98968)


  • Product name
    Anti-GABA A Receptor beta 3 antibody [N87/25]
    See all GABA A Receptor beta 3 primary antibodies
  • Description
    Mouse monoclonal [N87/25] to GABA A Receptor beta 3
  • Host species
  • Specificity
    Does not cross react with GABA A Receptor Beta 2 or Beta 1.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Fusion protein: APMDVHNEMN EVAGSVGDTR NSAISFDNSG IQYRKQSMPK EGHGRYMGDR SIPHKKTHLR RRSS, corresponding to amino acids 370-433 of Mouse GABA A Receptor beta 3

  • Positive control
    • Rat brain lysate
  • General notes

    The clone number has been updated from S87-25 to N87/25, both clone numbers name the same antibody clone.




Our Abpromise guarantee covers the use of ab98968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Sufficient for detection of GABA A Receptor beta 3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.


  • Function
    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
  • Involvement in disease
    Epilepsy, childhood absence 5
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.
  • Cellular localization
    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECA5 antibody
    • GABA alpha receptor beta-2 subunit antibody
    • GABA(A) receptor subunit beta-3 antibody
    • GABAA receptor beta 3 subunit antibody
    • GABAA receptor subunit beta 3 antibody
    • GABR B3 antibody
    • Gabrb3 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 3 antibody
    • Gamma aminobutyric acid receptor subunit beta 3 antibody
    • Gamma-aminobutyric acid receptor subunit beta-3 antibody
    • GBRB3_HUMAN antibody
    • MGC9051 antibody
    see all


  • ab98968 staining GABA A Receptor beta 3 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • Anti-GABA A Receptor beta 3 antibody [N87/25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size: 55 kDa

  • Overlay histogram showing SH-SY5Y cells stained with ab98968 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Urraca N  et al. Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons. Mol Autism 9:6 (2018). WB . Read more (PubMed: 29423132) »
  • Konermann A  et al. Verification of ?-Amino-Butyric Acid (GABA) Signaling System Components in Periodontal Ligament Cells In Vivo and In Vitro. Cell Mol Neurobiol 36:1353-1363 (2016). Read more (PubMed: 26865191) »

See all 5 Publications for this product

Customer reviews and Q&As

According to uniprot (link below) there are four isoforms of GabaA Beta3, all of which ab98968 is capable of detecting. Isoforms 1 and 2 are ˜54 kDa, isoform 3 is 47 kDa, and isoform 4 is 45 kDa. Since GabaA Beta3 is glycosylated, I would suspect the s...

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Thank you very much for your comprehension, it’s really appreciated.
Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of a...

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