Overview

  • Product name
    Anti-GABA antibody [5A9]
    See all GABA primary antibodies
  • Description
    Mouse monoclonal [5A9] to GABA
  • Specificity
    ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-FoFrmore details
  • Immunogen

    GABA coupled to BSA with glutaraldehyde

  • General notes


    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
  • Clonality
    Monoclonal
  • Clone number
    5A9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Relevance
    Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • Cellular localization
    Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • Alternative names
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

Anti-GABA antibody [5A9] images

  • ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).

    Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.

  • Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.

References for Anti-GABA antibody [5A9] (ab86186)

This product has been referenced in:
  • Dacks AM  et al. A characterization of the Manduca sexta serotonin receptors in the context of olfactory neuromodulation. PLoS One 8:e69422 (2013). IHC-P . Read more (PubMed: 23922709) »
  • Mao YT & Pallas SL Cross-modal plasticity results in increased inhibition in primary auditory cortical areas. Neural Plast 2013:530651 (2013). IHC . Read more (PubMed: 24288625) »

See all 2 Publications for this product

Product Wall

Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (Brain)
Specification
Brain
Username

Mr. Lee Sun

Verified customer

Submitted Oct 21 2016

GABA is a neurotransmitter and species is not applicable for this biochemical. Therefore any anti GABA antibody should work with samples of wide range of species.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

Submitted Oct 23 2014

Thank you for contacting us.

GABA is a neurotransmitter which is a small molecule; because of this species is not applicable for anti GABA antibodies - these antibodies will work irrespective of species.

I hope this information is hel...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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